AGCoL SyMAP Tour UA
BIO5
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Following is a quick tour of the many displays and queries available through SyMAP.

Contents

Management:

Project Manager

Chromosome Explorer

High-level views:

Circle

Dot Plot

Block

Detailed views:

2D - 3 Chr

2D - 2 Chr

2D Closeup

Base View

Analysis:

Query Interface

Query Result

Multiple Alignment

Project Manager

After the synteny database has been build (see System Guide), the synteny can be viewed from the manager. All projects in the database will be listed on the left panel.

Selecting projects on the left panel shows them on the right panel. A check mark in the Available Syntenies matrix indicates pairs that have computed synteny that can be viewed.

By selecting a cell with synteny, the Display for Selected Pair buttons are activated, which display synteny views for the two-genome.

The Display for All Pairs provides multi-genome synteny views for all projects in the matrix.

symapPlants

Chromosome Explorer

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The Chromosome Explorer provides multi-chromosome for multi-genome.

Multiple chromosomes can be selected from different species on the left-hand panel.

The default display is the Circle view shown. From here, one can select Dot Plot or 2D.

For Circle and Dot Plot, multiple chromosomes can be selected and viewed (as shown below).

For 2D, it is best to only have two or three chromosomes selected (as shown below).

explorer

Circle View

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The Circle view is available for two-genome and multi-chromosome.

The left image shows all chromosomes for cabbage and B.rapa. The right image shows Cabbage chr1, B.rapa chr2, and A.thal chr3 and chr4.

circle-2-genome circle 4 chr

Dot Plot

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The Dot Plot view is available for multi-genome and multi-chromosome.

The left image shows all chromosomes for cabbage, R.rapa and A.thal. The right image shows Cabbage chr1, B.rapa chr2, and A.thal chr3 and chr4. Synteny blocks are outlined in blue, and one can click to zoom further and arrive at a more detailed 2D display.

dotplot 3 genome dotplot-4chr

Block View

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The Block View is a two-genome view.

The synteny blocks are colored by chromosome (see color key at top).

block-2-genome Clicking a chromosome pops up a window of the chromosome as shown below. Clicking a block pops up a 2D display.

block-2chr

2D 3-Chr

This is a three-chromosome view.

The dark blue and purple down the center of the chromosomes are annotated exons, where blue is positive strand and purple is negative strand.

The brown and green lines between chromosomes are hit-wires connected the similar region on the two chromosomes. The brown are hit-wires with the same orientation on both connecting chromosomes and the green are different orientation. Only hits that are part of a synteny block are shown here.

2d-3chr

2D 2-Chr

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This is a two-chromosome view.

The A.thal Chr4 and Cabbage Chr1 were displayed, a region was selected and the right track flipped.

The genes are staggered if they overlap by more than 3 bases.

The thin rectangles near the edge of each track are the hit regions connected by the hit-wire.

2D Closeup

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The image on the right shows a smaller region with the Annotation option is turned on.

A gene can be right clicked to view a popup of extended information, including the exons and hits.

The hit-wire can be right clicked to view of popup of details of its information.

Base View

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It is possible to zoom in even further and see a basepair-alignment of the hits in the region. base view

Query Interface

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SyMAP includes powerful search features to help make the most of synteny information.

query

Query Results

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The query results table has full flexibility in column selection and ordering.

query result

Putative gene families (PgeneF): The hits are grouped into gene families.
The PgeneF number is a unique number assigned to the gene family.
The PgFSize number is how many unique instances are in the family.

Multiple alignment

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By selecting a one or more rows, the genes sequences will be aligned using MUSCLE (Edgar 2004 NAR:32).

Email Comments To: symap@agcol.arizona.edu