AGCoL SyMAP Tour UA
BIO5
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Following is a quick tour of the many displays and queries available through SyMAP.

Contents

Whole genome views
Circle
Dot Plot
Block
Chromosome Explorer
Circle
Dot Plot
2D
Base View
Query Interface
Query Result
Multiple Alignment
Click any image for a close-up view

Whole genome views

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SyMAP can be used to query and view multiple synteny genome pairs at once (2-3 is best). The synteny can be viewed from the Project Manager. All projects in the database will be listed on the left panel.

Selecting projects on the left panel shows them on the right panel. A check mark in the Available Syntenies matrix indicates pairs that have computed synteny that can be viewed. These can be viewed from the lower multi-genome Display for All Pairs options.

By selecting a cell with synteny, the Display for Selected Pair buttons are activated, which display synteny views for the two-genome.

The Project Manager
viewPlants
The Circle view shows two genomes.
circle-2-genome

The Dot plot can show two or more genomes where the image on the lower left shows three.
A cell can be clicked to view a chromosome x chromosome Dot plot, from which a 2D view can be displayed.

dotplot 2 genome dotplot cell

The Block view shows two genomes.
Clicking a chromosome shows a close-up of it, where a block can be clicked to view the 2D display of it.

block-2-genome block-chr

Chromosome Explorer

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The Chromosome Explorer provides multi-chromosome views for multi-genomes.

Multiple chromosomes can be selected from different species on the left-hand panel.

The default display is the Circle view shown. From here, one can select Dot Plot or 2D.

explorer explorer dot plot

2D 3-Chr

This is a three-chromosome view.

The dark blue and purple down the center of the chromosomes are annotated exons, where blue is positive strand and purple is negative strand.

The brown and green lines between chromosomes are hit-wires connecting similar regions on the two chromosomes. The brown are hit-wires with the same orientation on both connecting chromosomes and the green are different orientation. Only hits that are part of a synteny block are shown here.

2d-3chr

2D Closeup

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The image on the right shows a smaller region with the Annotation option is turned on.

A gene can be right clicked to view a popup of extended information, including the exons and hits.

The hit-wire can be right clicked to view of popup of details of its information.

Base View

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It is possible to zoom in even further and see a basepair-alignment of the hits in the region. base view

Query Interface

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SyMAP includes powerful search features to help make the most of synteny information.

query

Query Results

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The query results table has full flexibility in column selection and ordering.

query result

Multiple alignment

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By selecting a one or more rows, the genes sequences can be aligned using MUSCLE or MAFFT (Refs6,7).

multiple alignment

Email Comments To: symap@agcol.arizona.edu