Following is a quick tour of the many displays and queries available through SyMAP.
Contents
Project Manager
After the synteny database has been build
(see System Guide), the synteny can be viewed
from the manager. All projects in the database will be listed on the left panel.
Selecting projects on the left panel shows them on the right panel. A check mark in the
Available Syntenies matrix indicates pairs that have computed synteny that can be viewed.
By selecting a cell with synteny,
the Display for Selected Pair buttons are activated, which
display synteny views for the two-genome.
The Display for All Pairs provides multi-genome synteny views for all projects in the
matrix.
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The Chromosome Explorer provides multi-chromosome for multi-genome.
Multiple chromosomes can be selected
from different species on the left-hand panel.
The default display is the Circle view shown.
From here, one can select Dot Plot or 2D.
For Circle and
Dot Plot,
multiple chromosomes can be selected and viewed (as shown below).
For 2D,
it is best to only have two or three chromosomes selected (as
shown below).
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The Circle view is available for two-genome and multi-chromosome.
The left
image shows all chromosomes for cabbage and B.rapa.
The right image shows Cabbage chr1, B.rapa chr2, and A.thal chr3 and chr4.
The Dot Plot view is available for multi-genome and multi-chromosome.
The left image shows all chromosomes for cabbage, R.rapa and A.thal.
The right image shows Cabbage chr1, B.rapa chr2, and A.thal chr3 and chr4.
Synteny blocks are outlined in blue, and one can click to zoom further and
arrive at a more detailed 2D display.
The Block View is a two-genome view.
The synteny blocks are colored by chromosome (see color key at top).
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Clicking a chromosome pops up a window of the chromosome as shown below. Clicking
a block pops up a 2D display.
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2D 3-Chr
This is a three-chromosome view.
The dark blue and purple down the center of the chromosomes are annotated exons,
where blue is positive strand and purple is negative strand.
The brown and green lines between chromosomes are hit-wires connected the similar
region on the two chromosomes. The brown are hit-wires with the same orientation on both
connecting chromosomes and the green are different orientation.
Only hits that are part of a synteny block are shown here.
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This is a two-chromosome view.
The A.thal Chr4 and Cabbage Chr1 were displayed, a region was selected and the right track flipped.
The genes are staggered if they overlap by more than 3 bases.
The thin rectangles near the edge of each track are the hit regions connected by the hit-wire.
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The image on the right shows a smaller region with the Annotation option
is turned on.
A gene can be right clicked to view a popup of extended information,
including the exons and hits.
The hit-wire can be right clicked to view of popup of details of its information.
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It is possible to zoom in even further and see a basepair-alignment
of the hits in the region.
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SyMAP includes powerful search features to help make the most of synteny information.
The query results table has full flexibility in column selection and ordering.
Putative gene families (PgeneF): The hits are grouped into gene families.
The PgeneF number is a unique number assigned to the gene family.
The PgFSize number is how many unique instances are in the family.
By selecting a one or more rows, the genes sequences will be aligned using
MUSCLE (Edgar 2004 NAR:32).
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